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Sequenom iplex massarray multiplex assay platform
Iplex Massarray Multiplex Assay Platform, supplied by Sequenom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/massarray+multiplex+iplex+assay/sequenom+massarray/pm38002974-61-14-19
Average 90 stars, based on 1 article reviews
iplex massarray multiplex assay platform - by Bioz Stars, 2026-09
90/100 stars

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Multiplex Assay:

Article Title: Natural allelic diversity, genetic structure and linkage disequilibrium pattern in wild chickpea.
Article Snippet: .. The Sequenom MassARRAY multiplex iPLEX assay including pre-amplification primary forward and reverse PCR primers and one unextended primer (UEP) [act as single nucleotide extension primers (EP)] for each 384 SNP loci were designed using MassARRAY Assay Design software v3.1. ..

Article Title: An Integrated Genomic Approach for Rapid Delineation of Candidate Genes Regulating Agro-Morphological Traits in Chickpea
Article Snippet: Four hundred and seventy SSR markers showing polymorphism between parental accessions (ICC 4958 and ICC 17160) were polymerase chain reaction (PCR) amplified and genotyped in 229 RIL mapping individuals using the 3.5% metaphor agarose gel and fluorescent dye-labelled automated fragment analyzer following Kujur et al . , A set of 369 TF gene-derived SNP markers showing polymorphism between parents were further genotyped in RILs using Sequenom MALDI-TOF MassARRAY system ( http://www.sequenom.com ). .. The designing of MassARRAY multiplex iPLEX assay, amplification of synthesized pre-amplification primers with multiplex PCR assay, shrimp alkaline phosphate incubation, primer extension, resin clean up and mass spectrometry were performed following the manufacturer's instructions of Sequenom iPLEX Gold amplification kit ( http://www.sequenom.com ) and methods of Pandit et al. ..

Article Title: Natural Allelic Diversity, Genetic Structure and Linkage Disequilibrium Pattern in Wild Chickpea
Article Snippet: .. The Sequenom MassARRAY multiplex iPLEX assay including pre-amplification primary forward and reverse PCR primers and one unextended primer (UEP) [act as single nucleotide extension primers (EP)] for each 384 SNP loci were designed using MassARRAY Assay Design software v3.1. ..

Polymerase Chain Reaction:

Article Title: Natural allelic diversity, genetic structure and linkage disequilibrium pattern in wild chickpea.
Article Snippet: .. The Sequenom MassARRAY multiplex iPLEX assay including pre-amplification primary forward and reverse PCR primers and one unextended primer (UEP) [act as single nucleotide extension primers (EP)] for each 384 SNP loci were designed using MassARRAY Assay Design software v3.1. ..

Article Title: An Integrated Genomic Approach for Rapid Delineation of Candidate Genes Regulating Agro-Morphological Traits in Chickpea
Article Snippet: Four hundred and seventy SSR markers showing polymorphism between parental accessions (ICC 4958 and ICC 17160) were polymerase chain reaction (PCR) amplified and genotyped in 229 RIL mapping individuals using the 3.5% metaphor agarose gel and fluorescent dye-labelled automated fragment analyzer following Kujur et al . , A set of 369 TF gene-derived SNP markers showing polymorphism between parents were further genotyped in RILs using Sequenom MALDI-TOF MassARRAY system ( http://www.sequenom.com ). .. The designing of MassARRAY multiplex iPLEX assay, amplification of synthesized pre-amplification primers with multiplex PCR assay, shrimp alkaline phosphate incubation, primer extension, resin clean up and mass spectrometry were performed following the manufacturer's instructions of Sequenom iPLEX Gold amplification kit ( http://www.sequenom.com ) and methods of Pandit et al. ..

Article Title: Natural Allelic Diversity, Genetic Structure and Linkage Disequilibrium Pattern in Wild Chickpea
Article Snippet: .. The Sequenom MassARRAY multiplex iPLEX assay including pre-amplification primary forward and reverse PCR primers and one unextended primer (UEP) [act as single nucleotide extension primers (EP)] for each 384 SNP loci were designed using MassARRAY Assay Design software v3.1. ..

Software:

Article Title: Natural allelic diversity, genetic structure and linkage disequilibrium pattern in wild chickpea.
Article Snippet: .. The Sequenom MassARRAY multiplex iPLEX assay including pre-amplification primary forward and reverse PCR primers and one unextended primer (UEP) [act as single nucleotide extension primers (EP)] for each 384 SNP loci were designed using MassARRAY Assay Design software v3.1. ..

Article Title: Natural Allelic Diversity, Genetic Structure and Linkage Disequilibrium Pattern in Wild Chickpea
Article Snippet: .. The Sequenom MassARRAY multiplex iPLEX assay including pre-amplification primary forward and reverse PCR primers and one unextended primer (UEP) [act as single nucleotide extension primers (EP)] for each 384 SNP loci were designed using MassARRAY Assay Design software v3.1. ..

Amplification:

Article Title: An Integrated Genomic Approach for Rapid Delineation of Candidate Genes Regulating Agro-Morphological Traits in Chickpea
Article Snippet: Four hundred and seventy SSR markers showing polymorphism between parental accessions (ICC 4958 and ICC 17160) were polymerase chain reaction (PCR) amplified and genotyped in 229 RIL mapping individuals using the 3.5% metaphor agarose gel and fluorescent dye-labelled automated fragment analyzer following Kujur et al . , A set of 369 TF gene-derived SNP markers showing polymorphism between parents were further genotyped in RILs using Sequenom MALDI-TOF MassARRAY system ( http://www.sequenom.com ). .. The designing of MassARRAY multiplex iPLEX assay, amplification of synthesized pre-amplification primers with multiplex PCR assay, shrimp alkaline phosphate incubation, primer extension, resin clean up and mass spectrometry were performed following the manufacturer's instructions of Sequenom iPLEX Gold amplification kit ( http://www.sequenom.com ) and methods of Pandit et al. ..

Synthesized:

Article Title: An Integrated Genomic Approach for Rapid Delineation of Candidate Genes Regulating Agro-Morphological Traits in Chickpea
Article Snippet: Four hundred and seventy SSR markers showing polymorphism between parental accessions (ICC 4958 and ICC 17160) were polymerase chain reaction (PCR) amplified and genotyped in 229 RIL mapping individuals using the 3.5% metaphor agarose gel and fluorescent dye-labelled automated fragment analyzer following Kujur et al . , A set of 369 TF gene-derived SNP markers showing polymorphism between parents were further genotyped in RILs using Sequenom MALDI-TOF MassARRAY system ( http://www.sequenom.com ). .. The designing of MassARRAY multiplex iPLEX assay, amplification of synthesized pre-amplification primers with multiplex PCR assay, shrimp alkaline phosphate incubation, primer extension, resin clean up and mass spectrometry were performed following the manufacturer's instructions of Sequenom iPLEX Gold amplification kit ( http://www.sequenom.com ) and methods of Pandit et al. ..

Incubation:

Article Title: An Integrated Genomic Approach for Rapid Delineation of Candidate Genes Regulating Agro-Morphological Traits in Chickpea
Article Snippet: Four hundred and seventy SSR markers showing polymorphism between parental accessions (ICC 4958 and ICC 17160) were polymerase chain reaction (PCR) amplified and genotyped in 229 RIL mapping individuals using the 3.5% metaphor agarose gel and fluorescent dye-labelled automated fragment analyzer following Kujur et al . , A set of 369 TF gene-derived SNP markers showing polymorphism between parents were further genotyped in RILs using Sequenom MALDI-TOF MassARRAY system ( http://www.sequenom.com ). .. The designing of MassARRAY multiplex iPLEX assay, amplification of synthesized pre-amplification primers with multiplex PCR assay, shrimp alkaline phosphate incubation, primer extension, resin clean up and mass spectrometry were performed following the manufacturer's instructions of Sequenom iPLEX Gold amplification kit ( http://www.sequenom.com ) and methods of Pandit et al. ..

Mass Spectrometry:

Article Title: An Integrated Genomic Approach for Rapid Delineation of Candidate Genes Regulating Agro-Morphological Traits in Chickpea
Article Snippet: Four hundred and seventy SSR markers showing polymorphism between parental accessions (ICC 4958 and ICC 17160) were polymerase chain reaction (PCR) amplified and genotyped in 229 RIL mapping individuals using the 3.5% metaphor agarose gel and fluorescent dye-labelled automated fragment analyzer following Kujur et al . , A set of 369 TF gene-derived SNP markers showing polymorphism between parents were further genotyped in RILs using Sequenom MALDI-TOF MassARRAY system ( http://www.sequenom.com ). .. The designing of MassARRAY multiplex iPLEX assay, amplification of synthesized pre-amplification primers with multiplex PCR assay, shrimp alkaline phosphate incubation, primer extension, resin clean up and mass spectrometry were performed following the manufacturer's instructions of Sequenom iPLEX Gold amplification kit ( http://www.sequenom.com ) and methods of Pandit et al. ..



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